Publications
58. Synthesis of 19F MRI Nanotracers by Dispersion Polymerization-Induced Self-Assembly of N-(2,2,2-Trifluoroethyl)acrylamide in Water
Vyshakh M Panakkal, Dominik Havlicek, Ewa Pavlova, Marcela Filipová, Semira Bener, Daniel Jirak, Ondrej Sedlacek, Biomacromolecules 2022, 23, 11, 4814. https://doi.org/10.1021/acs.biomac.2c00981
57. Influence of Chain Length of Gradient and Block Copoly(2-oxazoline)s on Self-Assembly and Drug Encapsulation
Ondrej Sedlacek, Valentin Bardoula, Elina Vuorimaa‐Laukkanen, Lars Gedda, Katarina Edwards, Aurel Radulescu, Grigoriy A Mun, Yong Guo, Junnian Zhou, Hongbo Zhang, Véronique Nardello‐Rataj, Sergey Filippov, Richard Hoogenboom, Small 2022, 18, 17, 2106251. https://doi.org/10.1002/smll.202106251
56. Phase Separation and pH-Dependent Behavior of Four-Arm Star-Shaped Porphyrin-PNIPAM4 Conjugates
Nadiia Velychkivska, Ondřej Sedláček, Anastasiia B Shatan, Monika Spasovová, Sergey K Filippov, Mandeep K Chahal, Larisa Janisova, Jiří Brus, Lenka Hanyková, Jonathan P Hill, Francoise M Winnik, Jan Labuta, Macromolecules 2022, 2022, 55, 6, 2109. https://doi.org/10.1021/acs.macromol.1c02188
55. Thermoresponsive Triblock Copolymers as Widely Applicable 19F Magnetic Resonance Imaging Tracers
Kristyna Kolouchova, Ondrej Groborz, Miroslav Slouf, Vit Herynek, Laurens Parmentier, David Babuka, Zulfiya Cernochova, Filip Koucky, Ondrej Sedlacek, Martin Hruby, Richard Hoogenboom, Sandra Van Vlierberghe, Chemistry of Materials 2022, 2022, 34, 24, 10902. https://doi.org/10.1021/acs.chemmater.2c02589
54. Linear Poly(ethylenimine-propylenimine) Random Copolymers for Gene Delivery: From Polymer Synthesis to Efficient Transfection with High Serum Tolerance
M Rachèl Elzes, Ine Mertens, Ondrej Sedlacek, Bart Verbraeken, Aniek CA Doensen, Maarten A Mees, Mathias Glassner, Somdeb Jana, Jos MJ Paulusse, Richard Hoogenboom, Biomacromolecules 2022, 23, 6, 2459-2470. https://doi.org/10.1021/acs.biomac.2c00210
53. Fluorinated Ferrocene Moieties as a Platform for Redox-Responsive Polymer 19F MRI Theranostics
Pavel Švec, Oleg V Petrov, Jan Lang, Petr Štěpnička, Ondřej Groborz, David Dunlop, Jan Blahut, Kristýna Kolouchová, Lenka Loukotová, Ondřej Sedláček, Tomáš Heizer, Zdeněk Tošner, Miroslav Šlouf, Hynek Beneš, Richard Hoogenboom, Martin Hrubý, Macromolecules 2022, 2022, 55, 2, 658–671
52. Self-Assembly, Drug Encapsulation, and Cellular Uptake of Block and Gradient Copolymers of 2-Methyl-2-oxazine and 2-n-Propyl/butyl-2-oxazoline
David Babuka, Kristyna Kolouchova, Lenka Loukotova, Ondrej Sedlacek, Ondrej Groborz, Aneta Skarkova, Alexander Zhigunov, Ewa Pavlova, Richard Hoogenboom, Martin Hruby, Petr Stepanek, Macromolecules 2021, 54, 23, 10667
51. Antifouling fluoropolymer-coated nanomaterials for 19F MRI
Daniel Jirak, Jan Svoboda, Marcela Filipová, Ognen Pop-Georgievski, Ondrej Sedlacek , Chem. Commun. 2021, 2021, 57, 4718-4721
50. Fluorine-Containing Block and Gradient Copoly (2-oxazoline) s Based on 2-(3, 3, 3-Trifluoropropyl)-2-oxazoline: A Quest for the Optimal Self-Assembled Structure for 19F Imaging
Leonid I Kaberov, Zhansaya Kaberova, Anastasiia Murmiliuk, Jiri Trousil, Ondrej Sedlacek, Rafal Konefal, Alexander Zhigunov, Ewa Pavlova, Martin Vít, Daniel Jirák, Richard Hoogenboom, Sergey K Filippov, Biomacromolecules 2021, 22, 7, 2963–2975
49. A broad tuneable birdcage coil for mouse 1H/19F MR applications
M Vít, M Burian, Z Berková, J Lacik, O Sedlacek, Richard Hoogenboom, Z Raida, D Jirak, Journal of Magnetic Resonance 2021, 329, 107023
48. Fluorinated Water-Soluble Poly(2-oxazoline)s as Highly Sensitive 19F MRI Contrast Agents
Sedlacek, O.,* Jirak, D., Vit, M., Ziołkowska, N., Janouskova, O., and Hoogenboom, R.* Macromolecules 2020, 53, 15, 6387
Pre – Charles University publications
47. A unified kinetic Monte Carlo approach to evaluate (a)symmetric block and gradient copolymers with linear and branched chains illustrated for poly(2-oxazoline)s
Robert Conka, Yoshi W Marien, Ondrej Sedlacek, Richard Hoogenboom, Paul HM Van Steenberge, Dagmar R D’hooge, Polymer Chemistry 2022, 13, 1559-1575
46. Immiscibility of Chemically Alike Amorphous Polymers: Phase Separation of Poly(2-ethyl-2-oxazoline) and Poly(2-n-propyl-2-oxazoline)
Ella Schoolaert, Ronald Merckx, Jana Becelaere, Melissa Everaerts, Joachim FR Van Guyse, Ondrej Sedlacek, Bruno G De Geest, Guy Van den Mooter, Dagmar R D’hooge, Karen De Clerck, Richard Hoogenboom, Macromolecules 2020, 53, 17, 7590
45. Drug Delivery Systems Based on Poly(2‐Oxazoline)s and Poly (2‐Oxazine)s
Sedlacek, O.,* and Hoogenboom, R.* Adv. Ther. 2020, 3, 1900168
44. Poly (2-methyl-2-oxazoline) conjugates with doxorubicin: From synthesis of high drug loading water-soluble constructs to in vitro anti-cancer properties
Sedlacek, O.,* Van Driessche, A., Uvyn, A., De Geest, B., and Hoogenboom, R.* J. Control. Release 2020, 326, 53
43. Iodinated Choline Transport-Targeted Tracers
Pavel Švec, Zbyněk Nový, Jan Kučka, Miloš Petřík, Ondřej Sedláček, Martin Kuchař, Barbora Lišková, Martina Medvedíková, Kristýna Kolouchová, Ondřej Groborz, Lenka Loukotová, Rafał Ł Konefał, Marián Hajdúch, Martin Hrubý, J. Med. Chem. 2020, 63, 24, 15960–15978
42. Reactive oxygen species (ROS)-responsive polymersomes with site-specific chemotherapeutic delivery into tumors via spacer design chemistry
Jager, E., Sincari, V., Albuquerque, L., Jager, A., Humajova, J., Kucka, J., Pankrac, J., Paral, P., Heizer, T., Janouskova, O., Konefał, R., Pavlova, E., Sedlacek, O., Giacomelli, F.C., Pouckova, P., Sefc, L., Stepanek, P., and Hruby, M. Biomacromolecules 2020, 21 (4) 1437
41. Poly(2-ethyl-2-oxazoline) Conjugates with Salicylic Acid via Degradable Modular Ester Linkages
Bernhard, Y., Sedlacek, O., Van Guyse, J., Bender, J., Zhong, Z., De Geest, B. G., and Hoogenboom, R. Biomacromolecules 2020, 21, 8, 3207–3215
40. Implant-forming polymeric 19F MRI-tracer with tunable dissolution
Kolouchova, K., Jirak, D., Groborz, O., Sedlacek, O., Ziolkowska, N., Vit, M., Sticova, E., Galisova, A., Svec, P., Trousil, J., Hajek, M., and Hruby, M., J. Control. Release 2020, 327, 50-60
2019
39. Unexpected Reactivity Switch in the Statistical Copolymerization of 2-Oxazolines and 2-Oxazines Enabling the One-Step Synthesis of Amphiphilic Gradient Copolymers
Sedlacek, O., Lava, K., Verbraeken, B., Kasmi, S, De Geest, B., Hoogenboom, R. J. Am. Chem. Soc., 2019, 141, 24, 9617-9622
38. Straightforward route to new poly (2-oxazoline)s via acylation of well-defined polyethyleneimine
Sedlacek, O., Janouskova, O., Verbraeken, B., Hoogenboom, R. Biomacromolecules, 2019, 20, 1, 222-230
37. Poly (2-amino-2-oxazoline)s: a new class of thermoresponsive polymers
Sedlacek, O.*, Bera, D., Hoogenboom. R.* Polym. Chem., 2019, 10, 34, 4683-4689.
36. Visualization and design of the functional group distribution during statistical copolymerization
Van Steenberge, P., Sedlacek, O., Hernandez-Ortiz, J., Verbraeken, B., Reyniers, M.-F., Hoogenboom, R., D’hooge, D. Nat. Commun., 2019, 10, 1, 1-14.
35. Solvent-control over monomer distribution in the copolymerization of 2-oxazolines and the effect of a gradient structure on self-assembly
Bera, D., Sedlacek, O., Hoogenboom, R. Polym. Chem., 2019, 10, 37, 5116-5123
34. Poly(2-oxazoline)-protein conjugates
Sedlacek, O., Rosa., V., Hoogenboom, R. Eur. Polym. J., 2019, 120, 109246.
33. Synthesis of defined high molar mass poly(2-methyl-2-oxazoline)
Sedlacek, O.*, Monnery, B., Hogenboom, R.* Polym. Chem., 2019, 10, 11, 1276-1290
32. Poly(2-oxazoline)s One-Pot Polymerization and Surface Coating: From Synthesis to Antifouling Properties Out-Performing Poly(ethylene oxide)
Svoboda, J., Sedlacek, O., Riedel, T., Hruby, M., Pop-Georgievski, O. Biomacromolecules, 2019, 20, 9, 3453-3463.
31. SET‐LRP Synthesis of Well‐Defined Light‐Responsible Block Copolymer Micelles
Sedlacek, O.*, Filippov, S., Svec, P., Hruby, M. Macromol. Chem. Phys., 2019, 220, 19, 1900238
2018
30. Defined High Molar Mass Poly(2‐Oxazoline)s
Monnery, B., Jerca, V., Sedlacek, O., Verbraeken, B., Cavill, R., Hoogenboom, R. Angew. Chem. Int. Ed., 2018, 57, 47, 15400-15404
29. 19F Magnetic Resonance Imaging of Injectable Polymeric Implants with Multiresponsive Behavior
Sedlacek, O.*, Jirak, D.,* Galisova, A., Jager, E., Laaser, J., Lodge, T., Stepanek, P., Hruby, M.* Chem. Mater., 2018, 30, 15, 4892-4896
28. Self-Assembled Thermoresponsive Polymeric Nanogels for 19F MR Imaging
Kolouchova, K., Sedlacek, O., Jirak, D., Babuka, D., Blahut, J., Kotek, J., Vit, M., Trousil, J., Konefal, R., Janouskova, O., Podhorska, B., Slouf, M., Hruby., M. Biomacromolecules, 2018, 19, 8, 3515-3524
27. Biopolymer strategy for the treatment of Wilson’s disease
Vetrik, M., Mattova, J., Mackova, H., Kucka, J., Pouckova, P., Kukackova, O., Brus, J., Eigner-Henke, S., Sedlacek, O., Sefc, L., Stepanek, P., Hruby, M. J. Control. Release, 2018, 273, 131-138
26. Polymer nitric oxide donors potentiate the treatment of experimental solid tumours by increasing drug accumulation in the tumour tissue
Studenovsky, M., Sivak, L., Sedlacek, O., Konefal, R., Horkova, V., Etrych, T., Kovar, M., Rihova, B., Sirova, M. J. Control. Release, 2018, 269, 214-224
25. Porous heat-treated polyacrylonitrile scaffolds for bone tissue engineering
Vetrik, M., Parizek, M., Hadraba, D., Kukackova, O., Brus, J., Hlidkova, H., Komankova, L., Hodan, J., Sedlacek, O., Slouf, M., Bacakova, L., Hruby, M., ACS Appl. Mater. Interfaces, 2018, 10, 10, 8496-8506
24. Conformational properties of biocompatible poly (2-ethyl-2-oxazoline) s in phosphate buffered saline
Gubarev, A., Monnery, B., Lezov, A., Sedlacek, O., Tsvetkov, N., Hoogenboom, R., Filippov, S. Polym. Chem., 2018, 9, 17., 2232-2237
2017
23. Poly(2-ethyl-2-oxazoline) conjugates with doxorubicin for cancer therapy: in vitro and in vivo evaluation and direct comparison to poly [N-(2-hydroxypropyl) methacrylamide] analogues
Sedlacek, O.*, Monnery, B., Mattova, J., Kucka, J., Panek, J., Janouskova, O., Hocherl, A., Verbraeken, B., Vergaelen, M., Zadinova, M., Hoogenboom, R.,* Hruby, M.* Biomaterials, 2017, 146, 1-12
22. A simple neridronate-based surface coating strategy for upconversion nanoparticles: highly colloidally stable 125I-radiolabeled NaYF4: Yb3+/Er3+@ PEG nanoparticles for multimodal in vivo tissue imaging
Kostiv, U., Lobaz, V., Kucka, J., Svec, P., Sedlacek, O., Hruby, M., Janouskova, O., Francova, P., Kolarova, V., Sefc, L., Horak, D. Nanoscale, 2017, 9, 43, 16680-16688
21. Therapeutic targeting of non-coding RNAs in cancer
Slaby, O., Laga, R., Sedlacek, O. Biochem. J., 2017, 474, 24, 4219-4251
20. Reductively Degradable Poly (2-hydroxyethyl methacrylate) Hydrogels with Oriented Porosity for Tissue Engineering Applications
Mackova, H., Plichta, Z., Hlidkova, H., Sedlacek, O., Konefal, R., Sadakbayeva, Z., Duskova-Smrckova, M., Horak, D., Kubinova, S. ACS Appl. Mater. Interfaces, 2017, 9, 10544-10553
19. Radiolabelled Polymeric Materials for Imaging and Treatment of Cancer: Quo Vadis?
Pant, K., Sedlacek, O., Nadar, R., Hruby, M., Holger, S. Adv. Healthcare Mater., 2017, 6, 6, 1601115
18. The effect of ionizing radiation on biocompatible polymers: From sterilization to radiolysis and hydrogel formation
Sedlacek, O.*, Kucka, J., Monnery, B.D., Slouf, M., Vetrik, M., Hoogenboom, R., Hruby, M. Polym. Degrad. Stab., 2017, 137, 1-10
2016
17. Thermoresponsive polymers for nuclear medicine: Which polymer is the best?
Sedlacek, O., Cernoch, P., Kucka, J., Konefal, R., Stepanek, P., Vetrik, M., Lodge, T.P., Hruby, M. Langmuir, 2016, 32, 6115-6122
16. Double stimuli-responsive polymer systems: How to use crosstalk between pH-and thermosensitivity for drug depots
Bogomolova, A., Kaberov, L., Sedlacek, O., Filippov, S.K., Kral, V., Wang, X.Y., Liu, S.L., Ye, X.D., Hruby, M. Eur. Polym. J., 2016, 84, 54-64
2015
15. Optimized protocol for the radioiodination of hydrazone-type polymer drug delivery systems
Sedlacek, O., Kucka, J., Hruby, M. Appl. Radiat. Isot., 2015, 95, 129-134
14. Multi Responsive Polymer Micelles as Ellipticine Delivery Carriers for Cancer Therapy
Studenovsky, M., Sedlacek, O., Hruby, M., Panek, J., Ulbrich, K. Anticancer Res., 2015, 35 (2), 753-757
2014
13. Multistage-targeted pH-responsive polymer conjugate of Auger electron emitter: optimized design and in vivo activity
Sedláček, O., Studenovský, M., Větvička, D., Ulbrich, K., Hrubý, M. Bioorg. Med. Chem. 2013, 21 (18), 5669-5672
12. Glycogen-graft-poly(2-alkyl-2-oxazolines) – the new versatile biopolymer-based thermoresponsive macromolecular toolbox
Pospisilova A., Filippov, S.K, Bogomolova, A., Turner, S., Sedlacek, O., Matushkin, N., Cernochova, Z., Stepanek, P., Hruby, M. RSC Adv., 2014,4, 61580-61588
11. Silver-coated monolithic columns for scavenging of radioactive iodine
Sedlacek, O., Kucka, J., Svec, F., Hruby, M. J. Sep. Sci. 2014, 7 (37), 798-802
10. Study of Complex Thermosensitive Amphiphilic Polyoxazolines and Their Interaction with Ionic Surfactants. Are Hydrophobic, Thermosensitive and Hydrophilic Moieties Equally Important?
Bogomolova, A., Filippov, S., Starovoytova, L., Angelov, B., Konarev, P., Sedlacek, O., Hruby, M., Stepanek, P. J. Phys. Chem. B, 2014, 118 (18), 4940–4950
2013
9. Fine tuning of the pH-dependent drug release rate from polyHPMA-ellipticinium conjugates
Sedláček, O., Studenovský, M., Větvička, D., Ulbrich, K., Hrubý, M. Bioorg. Med. Chem. 2013, 21 (18), 5669-5672
8. SAXS and light scattering study of hybrid nanoparticles composed of thermoresponsive triblock copolymer F127 and thermoresponsive statistical poly-oxazolines with hydrophobic moieties
Bogomolova, A., Hruby, M., Panek, J., Rabyk, M., Turner, S., Bals, S., Steinhart, M., Zhigunov, A., Sedlacek, O., Stepanek, P., Filippov, S. J. Appl. Crystallogr. 2013, 46 (6), 1690-1698
7. Chelating polymeric particles intended for the therapy of Wilson’s disease
Škodová, M., Kučka, J., Vetrík, M., Skopal, J., Walterová, Z., Sedláček, O., Štěpánek, P., Mattová, J., Poučková, P., Urbánek, P., Hrubý, M. React. Funct. Polym. 2013, 73 (11), 1426-1431
6. Collective polyelectrolyte diffusion as a function of counterion size and dielectric constant
Filippov, S., Seery, T.,A., P., Kříž, J., Hrubý, M., Černoch, P., Sedláček, O., Kadlec, P., Pánek, J., Štěpánek, P. Polym. Int. 2013, 62 (9), 1271-1276
2012
5. Glycogen as Biodegradable Construction Nanomaterial for in vivo Use
Filippov, S., Sedlacek, O., Bogomolova, A., Vetrik, M., Jirak, D., Kovar, J., Kucka, J., Bals, S., Turner, S., Stepanek, P., Hruby, M. Macromol. Biosci. 2012, 12 (12), 1731-1738
4. Poly(2-oxazoline)s – where are they more advantageous for biomedical applications than other polymer types?
Sedláček, O.,Monnery B., D.,Filippov, S.,Hoogenboom, R.,Hrubý, M. Macromol. Rapid Commun. 2012, 33 (19), 1648-1662
3. Polymer conjugates of acridine-type anticancer drugs with pH-controlled activation
Sedláček, O., Hrubý, M., Studenovský, M., Větvička, D., Svoboda, J., Kaňková, D., Kovář, J., Ulbrich, K. Bioorg. Med. Chem. 2012, 20 (13), 4056-4063
2011
2. Ellipticine-aimed polymer-conjugated Auger electron emitter: multistage organelle targeting approach
Sedláček O., Hrubý M., Studenovský M., Kučka J., Větvicka D., Kovář L., Ulbrich K. Bioconjug. Chem. 2011, 22(6), 1194-1201
1. The synthesis of the 8-C-substituted 2,6-diamino-9-[2-(phosphonomethoxy)ethyl]purine (PMEDAP) derivatives by diverse cross-coupling reactions
Sedláček, O., Břehová, P., Pohl, R., Holý, A., Janeba, Z. Can. J. Chem. 2011, 89, 488-498